UV-Vis Spectroscopy

Instrument: Analytik Jena; Shimadzu UV1800

Overview

UV-vis spectroscopy measures the ability of a sample to absorb electromagnetic radiation in the visible-ultraviolet range to both confirm the presence of, and to quantify, coloured compounds in solution. Minimal sample preparation is required and the technique is a quick and reliable method to analyse coloured solutions.

Absorbance of UV light is also one of the preferred methods for quantifying both DNA and protein.

Technical Detail
Works well for...
Sample requirements